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Science Group Research Article Article ID: igmin217

DNA Genetics and UHPLC-Q-TOF-MS Analysis of Phytochemicals for Asparagus racemosus Roots

Spectroscopy Molecular BiologyBiochemistry
Nguyen Thi Huong * 1 ,
Do Ngoc Thuy 1 ,
Phung Van Trung 3 ,
Le Ngoc Hung 1,2 and
Mai Van Nam 4
Affiliation

Affiliation

    Center for High Technology Research and Development, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi, Vietnam

    Center for High Technology Research and Development, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi, Vietnam

    Center for High Technology Research and Development, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi, Vietnam

    Institute of Applied Materials Science, Vietnam Academy of Science and Technology, TL29 Str., Thanh Loc ward, Dist. 12, Ho Chi Minh City, Vietnam

    Center for High Technology Research and Development, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi, Vietnam | Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18-Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi, Vietnam

    Environmental and Equipment Technical Corporation, 7/52 Le Trong Tan, Thanh Xuan, Hanoi, Vietnam

DOI10.61927/igmin217 Fulltext HTML Fulltext PDF Cite this article
DOI10.61927/igmin217 Fulltext HTML Fulltext PDF Cite this article

Abstract

The medicinal herb Asparagus racemosus has been used in traditional medicine to treat various diseases such as cough, diarrhoea, diabetes, gastric issues, gonorrhoea, headaches, piles, rheumatism, and even lactation enhancement. This study explores the genetic information and phytochemicals of the species native to Gia Lai province, Vietnam before its conservation and cultivation. Five species of A. racemosus were analyzed using the trnL-e/trnL-f regions sequence. A. racemosus roots were extracted by water then Ultra-High-Performance Liquid Chromatography coupled with Quadrupole/Time-Of-Flight Mass Spectrometry (UHPLC-Q-TOF-MS) spectroscopy was used to screen for their phytochemicals. We have confirmed the DNA genetics of A. racemosus species collected in Gia Lai, Vietnam. In water extract of A. racemosus roots, UHPLC-Q-TOF-MS tentatively identified two flavonoids (Quercetin-3-glucuronide, Rutin), five steroidal saponins (Shatavarin I, Shatavarin IV, Shatavarin IX, Asparacoside, Asparanin A), and two steroids (β-sitosterol, Daucosterol). The experimental findings confirm the A. racemosus species for conservation and cultivation in Vietnam and contribute the benefits to the chemical literature of Vietnamese natural flora. A. racemosus should be further studied for pharmaceutical activities.

Figures

PCR product of DNA regions checked on 1% agarose gel. Abbreviations: 1, 2, 3, 4, 5, M: Marker 100bp plus DNA ladder, trnL-e/trnL-f (GGTTCAAGTCCCTCTATCCC/ATTTGAACTGGTGACACGAG). Figure 1: PCR product of DNA regions checked on 1% agarose g...
TIC of HPLC-ESI-Q-TOF-MS NEG mode of water extract of wild A. racemosus roots. Figure 2: TIC of HPLC-ESI-Q-TOF-MS NEG mode of water extract...
TIC of HPLC-ESI-Q-TOF-MS NEG mode of water extract of wild A. racemosus roots. Figure 3: TIC of HPLC-ESI-Q-TOF-MS NEG mode of water extract...
HPLC-ESI-Q-TOF NEG assignments of compounds in A. racemosus roots. Table 2: HPLC-ESI-Q-TOF NEG assignments of compounds in A. ...

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